H00005094-M07
antibody from Novus Biologicals
Targeting: PCBP2
hnRNP-E2, HNRNPE2, HNRPE2
Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00005094-M07 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005094-M07, RRID:AB_546797
- Product name
- Mouse Monoclonal PCBP2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PCBP2 - poly(rC) binding protein 2
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.
Poly(rC)-Binding Protein 2 Regulates Hippo Signaling To Control Growth in Breast Epithelial Cells.
Iron Export through the Transporter Ferroportin 1 Is Modulated by the Iron Chaperone PCBP2.
hnRNP L and NF90 interact with hepatitis C virus 5'-terminal untranslated RNA and promote efficient replication.
Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs.
Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.
Activation of the HIF prolyl hydroxylase by the iron chaperones PCBP1 and PCBP2.
Poly(C)-binding protein 2 interacts with sequences required for viral replication in the hepatitis C virus (HCV) 5' untranslated region and directs HCV RNA replication through circularizing the viral genome.
Identification of RNA helicase A as a new host factor in the replication cycle of foot-and-mouth disease virus.
Selective localization of PCBP2 to cytoplasmic processing bodies.
Rapid changes of mRNA-binding protein levels following glucose and 3-isobutyl-1-methylxanthine stimulation of insulinoma INS-1 cells.
Identification of PCBP2, a facilitator of IRES-mediated translation, as a novel constituent of stress granules and processing bodies.
A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress.
Roth H, Magg V, Uch F, Mutz P, Klein P, Haneke K, Lohmann V, Bartenschlager R, Fackler OT, Locker N, Stoecklin G, Ruggieri A
mBio 2017 Jan 10;8(1)
mBio 2017 Jan 10;8(1)
Poly(rC)-Binding Protein 2 Regulates Hippo Signaling To Control Growth in Breast Epithelial Cells.
Li F, Bullough KZ, Vashisht AA, Wohlschlegel JA, Philpott CC
Molecular and cellular biology 2016 Aug 15;36(16):2121-31
Molecular and cellular biology 2016 Aug 15;36(16):2121-31
Iron Export through the Transporter Ferroportin 1 Is Modulated by the Iron Chaperone PCBP2.
Yanatori I, Richardson DR, Imada K, Kishi F
The Journal of biological chemistry 2016 Aug 12;291(33):17303-18
The Journal of biological chemistry 2016 Aug 12;291(33):17303-18
hnRNP L and NF90 interact with hepatitis C virus 5'-terminal untranslated RNA and promote efficient replication.
Li Y, Masaki T, Shimakami T, Lemon SM
Journal of virology 2014 Jul;88(13):7199-209
Journal of virology 2014 Jul;88(13):7199-209
Active stabilization of human endothelial nitric oxide synthase mRNA by hnRNP E1 protects against antisense RNA and microRNAs.
Ho JJ, Robb GB, Tai SC, Turgeon PJ, Mawji IA, Man HS, Marsden PA
Molecular and cellular biology 2013 May;33(10):2029-46
Molecular and cellular biology 2013 May;33(10):2029-46
Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules.
Fujimura K, Sasaki AT, Anderson P
Nucleic acids research 2012 Sep;40(16):8099-110
Nucleic acids research 2012 Sep;40(16):8099-110
Activation of the HIF prolyl hydroxylase by the iron chaperones PCBP1 and PCBP2.
Nandal A, Ruiz JC, Subramanian P, Ghimire-Rijal S, Sinnamon RA, Stemmler TL, Bruick RK, Philpott CC
Cell metabolism 2011 Nov 2;14(5):647-57
Cell metabolism 2011 Nov 2;14(5):647-57
Poly(C)-binding protein 2 interacts with sequences required for viral replication in the hepatitis C virus (HCV) 5' untranslated region and directs HCV RNA replication through circularizing the viral genome.
Wang L, Jeng KS, Lai MM
Journal of virology 2011 Aug;85(16):7954-64
Journal of virology 2011 Aug;85(16):7954-64
Identification of RNA helicase A as a new host factor in the replication cycle of foot-and-mouth disease virus.
Lawrence P, Rieder E
Journal of virology 2009 Nov;83(21):11356-66
Journal of virology 2009 Nov;83(21):11356-66
Selective localization of PCBP2 to cytoplasmic processing bodies.
Fujimura K, Katahira J, Kano F, Yoneda Y, Murata M
Biochimica et biophysica acta 2009 May;1793(5):878-87
Biochimica et biophysica acta 2009 May;1793(5):878-87
Rapid changes of mRNA-binding protein levels following glucose and 3-isobutyl-1-methylxanthine stimulation of insulinoma INS-1 cells.
Süss C, Czupalla C, Winter C, Pursche T, Knoch KP, Schroeder M, Hoflack B, Solimena M
Molecular & cellular proteomics : MCP 2009 Mar;8(3):393-408
Molecular & cellular proteomics : MCP 2009 Mar;8(3):393-408
Identification of PCBP2, a facilitator of IRES-mediated translation, as a novel constituent of stress granules and processing bodies.
Fujimura K, Kano F, Murata M
RNA (New York, N.Y.) 2008 Mar;14(3):425-31
RNA (New York, N.Y.) 2008 Mar;14(3):425-31
A link between SIN1 (MAPKAP1) and poly(rC) binding protein 2 (PCBP2) in counteracting environmental stress.
Ghosh D, Srivastava GP, Xu D, Schulz LC, Roberts RM
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 19;105(33):11673-8
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 19;105(33):11673-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi or non-transfected control. Blot probed with H00005094-M07. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12. Lane 1: PCBP2 transfected lysatE (38.7 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of PCBP2 expression in K-562 (Cat # L009V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PCBP2 Antibody (5F12) [H00005094-M07] - Detection limit for recombinant GST tagged PCBP2 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCBP2 Antibody (5F12) [H00005094-M07] - Analysis of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml