PA5-30116

antibody from Invitrogen Antibodies

Targeting: PCBP2 hnRNP-E2, HNRNPE2, HNRPE2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30116

Provider

Invitrogen Antibodies

Product name

PCBP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, Raji, K562, NCI-H929. Predicted reactivity: Mouse (100%), Rat (97%), Pig (91%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.03 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Comprehensive profiling of mRNA splicing indicates that GC content signals altered cassette exon inclusion in Ewing sarcoma.

Graham GT, Selvanathan SP, Zöllner SK, Stahl E, Shlien A, Caplen NJ, Üren A, Toretsky JA
NAR cancer 2022 Mar;4(1):zcab052

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Internal Ribosome Entry Site Dramatically Reduces Transgene Expression in Hematopoietic Cells in a Position-Dependent Manner.

Zheng Q, Zhang X, Yang H, Xie J, Xie Y, Chen J, Yu C, Zhong C
Viruses 2019 Oct 8;11(10)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PCBP2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PCBP2 Polyclonal Antibody (Product # PA5-30116) diluted at 1:750. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PCBP2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PCBP2 Polyclonal Antibody (Product # PA5-30116) diluted at 1:750. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human muscle, using PCBP2 (Product # PA5-30116) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PCBP2 was performed in paraffin-embedded mouse brain tissue using PCBP2 Polyclonal Antibody (Product # PA5-30116) at a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 EMCV IRES inhibited the expression of transgene on the translational level. ( A ) HEK293 cells were transduced with rAAV6-CMVp- gfp at 10,000 vgs/cell. The relative rAAV6 genome content was detected by qPCR using GFP primers and ITR primers. ( B ) K562 and ( C ) HEK293 cells were transduced with rAAV6-CMVp- gfp and rAAV6-CMVp-EMCV IRES- gfp at 10,000 vgs/cell. Total DNA and RNA were isolated at 4 days post-transduction for qPCR. Transgene expression was detected by fluorescence microscopy at 72 hours post-transduction. (D) Western blot of total cell extracts (lysate) from HEK293 cells and K562 cells after rAAV6-CMVp- gfp or rAAV6-CMVp-EMCV IRES- gfp infection for Gemin5, PTBP1, and PCBP2 expression.