Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AM20926PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20926PU-N, RRID:AB_10842771
- Product name
- anti PCBP2 / hnRNP-E2
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein, PCBP2 (AAH01155, 1 a.a. ~ 363 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5F12
- Vial size
- 50 µg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody clone 5F12.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- PCBP2 monoclonal antibody clone 5F12 Western Blot analysis of PCBP2 expression in K-562.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCBP2 monoclonal antibody clone 5F12.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PCBP2 is approximately 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PCBP2 on HeLa cell (antibody concentration 10 µg/ml).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)