PA5-52913

antibody from Invitrogen Antibodies

Targeting: FCGRT alpha-chain, FCRN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52913

Provider

Invitrogen Antibodies

Product name

FCGRT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LTFLLFSCPH RLREHLERGR GNLEWKEPPS MRLKARPSSP GFSVLTCSAF SFYPPELQLR FLRNGLAAGT GQGDFGPNSD GSFHASSSLT VKSGDEHHYC CIVQHAGLAQ PLRVELESPA KS Highest antigen sequence identity to the following orthologs: Mouse - 72%, Rat - 73%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.15 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FCGRT in control (vector only transfected HEK293T lysate) and FCGRT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a FCGRT Polyclonal Antibody (Product # PA5-52913).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FCGRT in control (vector only transfected HEK293T lysate) and FCGRT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a FCGRT Polyclonal Antibody (Product # PA5-52913).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FCGRT in human cell lines Caco-2 and HeLa using a FCGRT Polyclonal Antibody (Product # PA5-52913). Corresponding FCGRT RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of FCGRT in human placenta using FCGRT Polyclonal Antibody (Product # PA5-52913) shows moderate to strong cytoplasmic positivity in Hofbauer cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of FCGRT in human liver using a FCGRT Polyclonal Antibody (Product # PA5-52913) shows moderate to strong cytoplasmic positivity in Kupffer cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of FCGRT in human cerebellum using a FCGRT Polyclonal Antibody (Product # PA5-52913) shows no positivity in neuronal cells as expected.