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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-97111 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FCGRT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HLSLLYHLTA VSSPAPGTPA FWVSGWLGPQ QYLSYNSLRG EAEPCGAWVW ENQVSWYWEK ETTDLRIKEK LFLEAFKALG GKGPYTLQGL LGCELGPDNT SVPTAKFALN GEEFMNFDLK QGTWGGDWPE ALAISQRWQQ QDKAANKELT FLLFSCPHRL REHLERGRGN LEWKEPPSMR LKARPSSPGF SVLTCSAFSF YPPELQLRFL RNGLAAGTGQ GDFGPNSDGS FHASSSLTVK SGDEHHYCCI VQHAGLAQPL RVEL Positive Samples: HL-60, Mouse brain, Mouse small intestine, Mouse liver, Mouse kidney; Cellular Location: Cell membrane, Single-pass type I membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.77 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FCGRT in extracts of HL-60 cells. Samples were incubated with FCGRT Polyclonal antibody (Product # PA5-97111) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FCGRT in A-549 cells. Samples were incubated with FCGRT Polyclonal antibody (Product # PA5-97111) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
