Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-17062 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CK2 alpha-2 Monoclonal Antibody (1E1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17062 targets CSNK2A2 in FACS, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples. The MA5-17062 immunogen is purified recombinant fragment of human CSNK2A2 (amino acids: 194-350) expressed in E. Coli. MA5-17062 detects CSNK2A2 which has a predicted molecular weight of approximately 41.2kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E1
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of potential candidate proteins for reprogramming spinal cord-derived astrocytes into neurons: a proteomic analysis.
Chen WH, Lin YX, Lin L, Zhang BQ, Xu SX, Wang W
Neural regeneration research 2021 Nov;16(11):2257-2263
Neural regeneration research 2021 Nov;16(11):2257-2263
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CSNK2A2 using a CSNK2A2 monoclonal antibody (Product # MA5-17062) against a human CSNK2A2 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CSNK2A2 using a CSNK2A2 monoclonal antibody (Product # MA5-17062) against a human CSNK2A2 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CSNK2A2 using CSNK2A2 monoclonal antibody (Product # MA5-17062) in HeLa (1), MCF-7 (2), HepG2 (3), Jurkat (4), NIH3T3 (5), and PC-12 (6) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using CSNK2A2 monoclonal antibody (Product # MA5-17062) (green) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Western blot assay to validate the key proteins involved in the reprogramming process and to compare protein expression between the GFP and NEUROD1 groups. Data are expressed as the mean +- SD. The experiment was repeated three times. * P < 0.05 (unpaired Student's t -test). CSNK2A2: Casein kinase II subunit alpha; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent protein; NEUROD1: neurogenic differentiation factor 1; PNN: pinin.
