ARG55840

antibody from Arigo

Targeting: AGR2 AG2, HAG-2, PDIA17, XAG-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG55840

Provider

Arigo

Product name

anti-AGR2 antibody

Antibody type

Polyclonal

Antigen

KLH-conjugated synthetic peptide around aa. 13-42 (N-terminus) of Human AGR2

Description

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Storage

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: 35 µg of HT29, MCF-7, ZR-75-1 cell line lysates stained with ARG55840 anti-AGR2 antibody.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: A549 cell stained with ARG55840 anti-AGR2 antibody (green) at 1:25 dilution, 1 h at 37°C. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARG55840 anti-AGR2 antibody. Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG55840 anti-AGR2 antibody.