Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-85636 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AGR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (93%), Rat (93%), Zebrafish (82%), Pig (96%), Rhesus Monkey (99%), Bovine (95%). Positive Control: MCF-7. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.203 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AGR2 in whole cell extract using AGR2 polyclonal antibody (Product # PA5-85636) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AGR2 Polyclonal Antibody (Product # PA5-85636). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with AGR2 Polyclonal Antibody (Product # PA5-85636) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGR2 protein stained by AGR2 antibody (Product # PA5-85636) diluted at 1:1,000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AGR2 antibody detects AGR2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. AGR2 antibody (Product # PA5-85636) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of AGR2 in MCF-7 whole cell extracts with AGR2 polyclonal antibody (Product # PA5-85636) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
