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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab089031 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Anterior Gradient 2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Anterior Gradient 2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein Function:Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 27-40- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,4 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Anterior Gradient 2 Antibody (Ab089031) - Western Blot All lanes: Recombinant Anterior Gradient 2 Antibody (Ab089031) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 16 kDa Exposure time: 25.6 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Anterior Gradient 2 Antibody (Ab089031) - IF/ICC Immunocytochemistry analysis of Anterior Gradient 2 (green) in MCF7 using Recombinant Anterior Gradient 2 Antibody (Ab089031), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Anterior Gradient 2 Antibody (Ab089031) - IHC Immunohistochemistry analysis of paraffin-embedded colon using Recombinant Anterior Gradient 2 Antibody (Ab089031). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
