HPA027247

antibody from Atlas Antibodies

Targeting: SLC9A3R1 EBP50, NHERF, NHERF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA027247

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027247, RRID:AB_10601162

Product name

Anti-SLC9A3R1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLP
VPFTNGEIQKENSREALAEAALESPRPALVRSASS
DTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNIS
LAMAKERAHQKRSSKRAPQMDWSKKNELFSNL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and SLC9A3R1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401364).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line MCF-7.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & microtubule organizing center.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using Anti-SLC9A3R1 antibody. Corresponding SLC9A3R1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human endometrium, kidney, skeletal muscle and small intestine using Anti-SLC9A3R1 antibody HPA027247 (A) shows similar protein distribution across tissues to independent antibody HPA009672 (B).

Antibody #2 product nr

HPA009672

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows distinct cytoplasmic and membranous positivity in tubular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-SLC9A3R1 antibody HPA027247.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium using Anti-SLC9A3R1 antibody HPA027247.

Sample type

HUMAN