Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A302-974A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NHERF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NHERF1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody (Product # A302-974A) used at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NHERF1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody A302-974A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NHERF1 was also immunoprecipitated by rabbit anti-NHERF1 antibodies A302-973A and A302-975A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NHERF1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NHERF1 (Cat. No. A302-974A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NHERF1 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-NHERF1 antibody (Product # A302-974A) used for WB at 1 µg/mL and for IP at 3 µg/mg lysate. NHERF1 was also immunoprecipitated by rabbit anti-NHERF1 antibody (Product # A302-975A). Detection: Chemiluminescence with exposure time of 3 seconds.
