18969-1-AP

antibody from Invitrogen Antibodies

Targeting: DPYSL3 CRMP4, DRP-3, ULIP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18969-1-AP

Provider

Invitrogen Antibodies

Product name

CRMP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EGHVLSRPE ELEAEAVFRA ITIASQTNCP LYVTKVMSKS AADLISQARK KGNVVFGEPI TASLGIDGTH YWSKNWAKAA AFVTSPPLSP DPTTPDYINS LLASGDLQLS GSAHCTFSTA QKAIGKDNFT AIPEGTNGVE ERMSVIWDKA VATGKMDENQ FVAVTSTNAA KIFNLYPRKG RISVGSDSDL VIWDPDAVKI VSAKNHQSAA EYNIFEGMEL RGAPLVVICQ GKIMLEDGNL HVTQGAGRFI PCSPFSDYVY KRIKARRKMA DLHAVPRGMY DGPVFDLTTT PKGGTPAGSA RGSPTRPNPP VRNLHQSGFS LSGTQVDEGV RSASKRIVAP PGGRSNITSL S (221-570 aa encoded by BC039006)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18969-1-AP (CRMP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using DPYSL3 antibody 18969-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18969-1-AP (CRMP4 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18969-1-AP (CRMP4 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 18969-1-AP ( CRMP4 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 18969-1-AP ( CRMP4 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CRMP4 (IP: 18969-1-AP, 3 µg; Detection: 18969-1-AP 1:700) with SH-SY5Y cells lysate 3000 µg.