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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21716 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UTP6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116. Predicted reactivity: Mouse (82%), Rhesus Monkey (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Global Interactomics Connect Nuclear Mitotic Apparatus Protein NUMA1 to Influenza Virus Maturation.
Rahim MN, Klewes L, Zahedi-Amiri A, Mai S, Coombs KM
Viruses 2018 Dec 19;10(12)
Viruses 2018 Dec 19;10(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UTP6 Polyclonal Antibody (Product # PA5-21716). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. UTP6 Polyclonal Antibody (Product # PA5-21716) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UTP6 in paraformaldehyde-fixed HeLa cells using a UTP6 polyclonal antibody (Product # PA5-21716) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (red) at a 1:2500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using UTP6 antibody (Product # PA5-21716) (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (red) at 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UTP6 Polyclonal Antibody detects UTP6 protein at nucleus on U251 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U251 xenograft. UTP6 Polyclonal Antibody (Product # PA5-21716) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
