Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C185520 - Provider product page
- Provider
- LSBio
- Product name
- STI1 / STIP1 Antibody (aa1-300) LS-C185520
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate). A: Hep G2, B: MOLT4. 7.5% SDS PAGE. STI1 / STIP1 antibody diluted at 1:5000
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- STIP1 antibody detects STIP1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 7.5 % SDS-PAGE. STIP1 antibody dilution:1:1000
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using STIP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using STIP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Cal27 xenograft using STIP1 antibody at 1:100 dilution.