AF7337

antibody from Novus Biologicals

Targeting: STIP1 HOP, STI1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF7337

Provider

Novus Biologicals

Product name

Sheep Polyclonal STI1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, rat STI1 in Western blots and detects recombinant human STI1 in direct ELISAs.

Reactivity

Human, Mouse, Rat

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Exploitation of the Leishmania exosomal pathway by Leishmania RNA virus 1.

Atayde VD, da Silva Lira Filho A, Chaparro V, Zimmermann A, Martel C, Jaramillo M, Olivier M
Nature microbiology 2019 Apr;4(4):714-723

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat STI1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat STI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7337) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STI1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.