PA5-89626

antibody from Invitrogen Antibodies

Targeting: STIP1 HOP, STI1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89626

Provider

Invitrogen Antibodies

Product name

STIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MEQVNELKEK GNKALSVGNI DDALQCYSEA IKLDPHNHVL YSNRSAAYAK KGDYQKAYED GCKTVDLKPD WGKGYSRKAA ALEFLNRFEE AKRTYEEGLK HEANNPQLKE GLQNMEARLA ERKFMNPFNM PNLYQKLESD PRTRTLLSDP TYRELIEQLR NKPSDLGTKL QDPRIMTTLS VLLGVDLGSM DEEEEIATPP PPPPPKKETK PEPMEEDLPE NKKQALKEKE LGNDAYKKKD FDTALKHYDK AKELDPTNMT YITNQAAVYF EKGDYNKCRE LCEKAIEVGR ENREDYRQIA; Positive Samples: HeLa, COS-7, COS-1, OVCAR3, Mouse brain; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.23 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of STIP1 was performed in HeLa cells using STIP1 Polyclonal Antibody (Product # PA5-89626).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of STIP1 in HeLa cells. Samples were incubated with STIP1 Polyclonal antibody (Product # PA5-89626). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of STIP1 in 200 μg extracts of HeLa cells. Samples were precipitated with 1 μg STIP1 Polyclonal antibody (Product # PA5-89626). Western blot was performed from the immunoprecipitate using STIP1 Polyclonal antibody (Product # PA5-89626) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STIP1 in paraffin-embedded rat testis. Samples were incubated with STIP1 Polyclonal antibody (Product # PA5-89626) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of STIP1 in paraffin-embedded mouse testis. Samples were incubated with STIP1 Polyclonal antibody (Product # PA5-89626) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of STIP1 was performed in 200 µg extracts of HeLa cells using STIP1 Polyclonal Antibody (Product # PA5-89626). Western blot was performed from the immunoprecipitate using STIP1 Polyclonal Antibody at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of STIP1 was performed in 200 µg extracts of HeLa cells using STIP1 Polyclonal Antibody (Product # PA5-89626). Western blot was performed from the immunoprecipitate using STIP1 Polyclonal Antibody at a dilution of 1:1000.