PA5-21473

antibody from Invitrogen Antibodies

Targeting: STIP1 HOP, STI1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21473

Provider

Invitrogen Antibodies

Product name

STIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, Molt-4, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12. Predicted reactivity: Mouse (95%), Rat (96%), Xenopus laevis (84%), Chimpanzee (100%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

STIP1 Polyclonal Antibody detects STIP1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.7.5 % SDS-PAGE. STIP1 Polyclonal Antibody (Product # PA5-21473) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using STIP1 Polyclonal Antibody (Product # PA5-21473). Sample (30 µg of whole cell lysate). A: Hep G2. B: MOLT4SDS PAGE. Z. 7.5% SDS PAGE. STIP1 Polyclonal Antibody (Product # PA5-21473) diluted at 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

STIP1 Polyclonal Antibody detects STIP1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.7.5 % SDS-PAGE. STIP1 Polyclonal Antibody (Product # PA5-21473) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of STIP1 in paraformaldehyde-fixed HeLa cells using a STIP1 polyclonal antibody (Product # PA5-21473) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1 antibody (Product # PA5-21473) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using STIP1 (Product # PA5-21473) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.