Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-52358 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAMP8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED LEATSEHFKT TSQKVARKFW WKNVKMI Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 92%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAMP8 in human cell line HL-60 using a VAMP8 Polyclonal Antibody (Product # PA5-52358).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-VAMP8 Polyclonal Antibody (Product # PA5-52358) and 11.4 kDa band corresponding to VAMP8 was observed in U937, Raji, Mouse Lung but not in SH-SY5Y, IMR32 and Mouse Brain which are reported to be negative. Whole cell extracts (30 µg lysate) of U937 (Lane 1), Raji (Lane 2), SH-SY5Y (Lane 3), IMR32 (Lane 4), tissue extracts (30 µg lysate) of Mouse Lung (Lane 5) and Mouse Brain (Lane 6) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of VAMP8 in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a VAMP8 Polyclonal Antibody (Product # PA5-52358).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of VAMP8 in human placenta using VAMP8 Polyclonal Antibody (Product # PA5-52358) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of VAMP8 in human cerebral cortex using VAMP8 Polyclonal Antibody (Product # PA5-52358) shows low expression as expected.