Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-70438 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAMP8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-VAMP8 Polyclonal Antibody (Product # PA5-70438) and a 11.4kDa band corresponding to VAMP8 was observed in U-937 and Mouse Lung, but was low in expression in all other tested models. Whole cell lysate (30ug lysate) of U-937 (Lane 1), OVCAR-3 (Lane 2), PANC-1 (Lane 3), SH-SY5Y (Lane 4), IMR-32 (Lane 5), Mouse Lung (Lane 6) and Mouse Brain (Lane 7) were electrophoresed using Novex™ 16% Tricine Protein Gels, 1.0 mm, 10-well (Product # EC6695BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).