Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF5354 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VAMP-8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human VAMP-8 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9BV40
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8.
SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor.
Madhavan SM, O'Toole JF, Konieczkowski M, Barisoni L, Thomas DB, Ganesan S, Bruggeman LA, Buck M, Sedor JR
JCI insight 2017 Jul 20;2(14)
JCI insight 2017 Jul 20;2(14)
SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor.
Zhu Q, Yamakuchi M, Lowenstein CJ
PloS one 2015;10(8):e0118737
PloS one 2015;10(8):e0118737
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human VAMP-8 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-8 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VAMP-8 in Human Pancreas. VAMP-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.