Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-113289 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SGMS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SGMS2 in rat liver tissue using a SGMS2 polyclonal antibody (Product # PA5-113289) at a concentration of 3 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SGMS2 in rat liver tissue using a SGMS2 polyclonal antibody (Product # PA5-113289) at a concentration of 3 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of SGMS2 in Hela cells using a Polyclonal antibody (Product # PA5-113289) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of SGMS2 in Hela cells using a Polyclonal antibody (Product # PA5-113289) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded SGMS2 in human appendix tissue using a SGMS2 Polyclonal antibody (Product # PA5-113289) at a dilution of 1:600.