32-0300

antibody from Invitrogen Antibodies

Targeting: GRIA2 GluA2, GLUR2, GLURB

Provider product page for 32-0300

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [25]
Comments [0]
Validations
Immunocytochemistry [1]
Flow cytometry [1]
Other assay [10]

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Product number: 32-0300 - Provider product page
Provider: Invitrogen Antibodies
Product name: GluR2 Monoclonal Antibody (6C4)
Antibody type: Monoclonal
Antigen: Other
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 6C4
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: -20°C

GRIA2 protein structure - 32-0300 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 GluA2 expression in LTP-stimulated rats. (a) Representative Western blot showing GluA2 (upper) and tubulin (lower) expression in laser microdissected tissue from one LTP-stimulated animal. (b) Mean relative expression of GluA2 in the molecular layer zones from LTP-stimulated and naive hemispheres, determined as a proportion of the ipsilateral inner molecular layer. (c) Mean percentage difference in tubulin-normalised expression of GluA2 in molecular layer zones of the LTP-stimulated hemisphere compared to the naive hemisphere; + p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Expression of subunits forming AMPAR (GluA1, GluA2) and NMDAR (GluN2A, GluN2B) in neurons of the cerebral cortex derived from Satb-deficient, Satb-, and control mice. ( A , B )--Immunostaining of GluA1 (green color), GluA2 (red color) subunits of AMPA receptors ( A ) and GluN2A (green color), GluN2B (red color) of NMDA receptors ( B ) of cortical neurons from control (Satb +/+ * Nex Cre/+ ), Satb-deficient (Satb fl/+ * Nex Cre/+ ) and Satb- (Satb2 fl/fl * Nex Cre/+ ) mice. ( C - F) --The effects of Satb- and Satb2-deletions on the level of GluA1 ( C ), GluA2 ( D ), GluN2A ( E ), and GluN2B ( F ) subunits. Intensity levels of surface-expressed receptor subunits were determined by confocal imaging. We analyzed individual neurons which had fluorescence of Alexa Fluor 633 (GluA1, green color), Alexa Fluor 488 (GluA2, red color) and Alexa Fluor-555 (GluN2B, red color), and Alexa Fluor-594 (GluN2A, green color). The quantitative data reflecting the level of subunits expression are presented as fluorescence intensity values in summary bar charts (mean +- SEM). The values were averaged by 300 +- 50 neurons for each column. Statistical significance was assessed using paired t-test. The results obtained after immunostaining well agree with the data of fluorescent Ca 2+ measurements presented in Figure 4 and Figure 5 . After Ca 2+ measurements, the cells were fixed and stained by the antibodies. We used the scans from three independent view fields for each experimental

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4. Altered composition of synaptic proteins in the PK2 E8Q/E8Q mice. ( A to C ) Western blot analysis of PCP components, regulator of PCP signaling, Mink1, alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)/N-methyl-D-aspartate receptor (NMDAR) subunits, and MAGUK family proteins in the crude synaptosomes of cortex and HPC from P14 PK2 E8Q/E8Q mice and WT littermates. ( D to F ) Quantitation of protein levels shown in (A) to (C). Each data point represents one animal. Data were normalized by dividing each value to the maximum value in each independent experiment. PK2 +/+ , n = 6; PK2 E8Q/E8Q , n = 6, Mann-Whitney U test, * P < 0.05 and ** P < 0.01. Error bars represent SEM. ( G to L ) Immunofluorescence staining of PK2 and Vangl2 in CA1 SR of P14 mice. To quantify the number (H) and the average size (I) of PK2 puncta, 66 image stacks of CA1 SR were taken from three PK2 +/+ animals, 66 image stacks from three PK2 E8Q/+ animals, and 68 image stacks from three PK2 E8Q/E8Q animals. To quantify the number (K) and the average size (L) of Vangl2 puncta, 62 image stacks were taken from three PK2 +/+ animals, 36 image stacks from two PK2 E8Q/+ animals, and 63 image stacks from PK2 E8Q/E8Q animals. Data were normalized by dividing each value to the maximum value in each independent experiment. Scale bars, 5 mum. Kruskal-Wallis test was used to compare the means of three groups, and Dunn's test was used to perform multiple comparisons. ** P < 0.01, *** P < 0