Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-38134 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-GluR2 (Ser880) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats.
Kim JE, Lee DS, Park H, Kim TH, Kang TC
Biomedicines 2021 Apr 14;9(4)
Biomedicines 2021 Apr 14;9(4)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-GluR2 pSer880 in extracts from mouse brain cells using a Phospho-GluR2 pSer880 polyclonal antibody (Product # PA5-38134).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Lung (Lane 3), Mouse Kidney (Lane 4), Rat Lung (Lane 5) and Rat Kidney (Lane 6). The blot was probed with anti-PhosphoGluR2 (Ser880) Polyclonal Antibody (Product # PA5-38134, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 100 kDa band corresponding to PhosphoGluR2 (Ser880) was observed in Mouse Brain and Rat Brain in comparison to other tissues which are reported negative for PhosphoGluR2 (Ser880) expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-GluR2 pSer880 in paraffin-embedded human brain tissue using a Phospho-GluR2 pSer880 polyclonal antibody (Product # PA5-38134).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 The effects of perampanel (PER) and GYKI 52466 (GYKI) on total glutamate ionotropic receptor AMPA type subunit 2 (GRIA2) expression and its phosphorylations in chronic epilepsy rats. ( A ) Representative images for Western blot of GRIA2 and its phosphorylation levels in the hippocampal tissues. ( B - F ) Quantifications of GRIA2 ( B ), p-GRIA2 Y869/Y873/Y876 ( C ), p-GRIA2 Y869/Y873/Y876/GRIA2 ratio ( D ), p-GRIA2 S880 ( E ), and p-GRIA2 S880/GRIA2 ratio ( F ) in the hippocampal tissues. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM ( *, # p < 0.05 vs. control (Cont) and vehicle (Veh)-treated animals, respectively; one-way ANOVA with post hoc Bonferroni''s multiple comparison). ( G - I ) Linear regression analyses between p-GRIA2 Y869/Y873/Y876/GRIA2 ratio and total seizure frequency ( G ), total seizure duration ( H ), and seizure severity ( I ) in chronic epilepsy rats.
