Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-89158 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89158, RRID:AB_11030327
- Product name
- Rabbit Polyclonal ALAD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, rat ALAD antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Heme Biosynthetic Pathway is Functionally Linked to Adipogenesis via Mitochondrial Respiratory Activity.
Moreno-Navarrete JM, Rodríguez A, Ortega F, Becerril S, Girones J, Sabater-Masdeu M, Latorre J, Ricart W, Frühbeck G, Fernández-Real JM
Obesity (Silver Spring, Md.) 2017 Oct;25(10):1723-1733
Obesity (Silver Spring, Md.) 2017 Oct;25(10):1723-1733
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALAD Antibody [NBP1-89158] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Liver
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ALAD Antibody [NBP1-89158] - Simple Western lane view shows a specific band for ALAD in 0.2 mg/ml of h. Liver (left) and h. Adrenal (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ALAD Antibody [NBP1-89158] - Electropherogram image of the corresponding Simple Western lane view. ALAD antibody was used at 1:200 dilution on h. Liver and h. Adrenal lysate(s) respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158] - Staining of human adrenal gland shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALAD Antibody [NBP1-89158] - Staining in human adrenal gland and pancreas tissues using anti-ALAD antibody. Corresponding ALAD RNA-seq data are presented for the same tissues.