PAB23260

antibody from Abnova Corporation

Targeting: HOXC11 HOX3H

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23260

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23260, RRID:AB_11123131

Product name

HOXC11 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant HOXC11.

Antigen sequence

STVSSFLPQAPSRQISYPYSAQVPPVREVSYGLEP
SGKWHHRNSYSSCYAAADELMHRECLPPSTVTEIL
MKNEGSYGGHHHPSAPHATPAGFYSSVNKNSVLP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with HOXC11 polyclonal antibody (Cat # PAB23260) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with HOXC11 polyclonal antibody (Cat # PAB23260) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with HOXC11 polyclonal antibody (Cat # PAB23260) shows strong cytoplasmic and nuclear positivity in purkinje cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)