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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- TA502575 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502575, RRID:AB_11125499
- Product name
- HOXC11 mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)
- Antibody type
- Monoclonal
- Description
- HOXC11 mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HOXC11
- Isotype
- IgG
- Antibody clone number
- OTI2H9
- Vial size
- 100 µl
- Concentration
- 0.28 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HOXC11 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC11 (RC201475, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC11.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HOXC11 mouse monoclonal antibody (TA502575) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HOXC11(RC201475).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HOXC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502575)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HOXC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502575)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201475 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HOXC11 antibody(TA502575), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-HOXC11 antibody(TA502575),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
