CF502572

antibody from Invitrogen Antibodies

Targeting: HOXC11 HOX3H

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF502572

Provider

Invitrogen Antibodies

Product name

HOXC11 Monoclonal Antibody (OTI3E10), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3E10

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC11 (RC201475, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC1. Positive lysates LY415427 (100 µg) and LC415427 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC11 (RC201475, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC1. Positive lysates LY415427 (100 µg) and LC415427 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (10 µg) from 9 Human tissue by using anti-HOXC11 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (10 µg) from a mouse cell line and 3 different mouse tissues by using anti-HOXC11 monoclonal antibody. (1:200)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-HOXC11 mouse monoclonal antibody (TA502572) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HOXC11(RC201475).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-HOXC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502572)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HOXC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502572)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HOXC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502572)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human tonsil within the normal limits using anti-HOXC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502572)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HOXC11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502572)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either RC201475 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HOXC11 antibody (TA502572), and then analyzed by flow cytometry.