PA5-29078

D2S1471, VIL

Antibody data

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Validation data

Product number: PA5-29078
Provider: Invitrogen Antibodies
Product name: Anti-Villin Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-29078
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: PA5-29078 targets Villin in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-29078 immunogen is recombinant fragment corresponding to a region within amino acids 7 and 286 of Human Villin. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Villin using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Villin polyclonal antibody (Product # PA5-29078) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Villin was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Villin Polyclonal Antibody (Product # PA5-29078) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Villin Polyclonal Antibody (Product # PA5-29078) and a 92kDa band corresponding to Villin was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), COLO 205 (Lane 2), HT-29 (Lane 3), Jurkat (Lane 4) and PANC-1 (Lane 5) as seen in Fig (a).Tissue extracts of Mouse Liver (Lane 1), Mouse Colon (Lane 2) and Mouse Jejunum (Lane 3) as seen in Fig (b) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).