Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [5]
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- Product number
- PA5-29078 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Villin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HepG2, mouse kidney. Predicted reactivity: Mouse (89%), Rat (89%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.36 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.
Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip.
Jing B, Wang ZA, Zhang C, Deng Q, Wei J, Luo Y, Zhang X, Li J, Du Y
Frontiers in bioengineering and biotechnology 2020;8:272
Frontiers in bioengineering and biotechnology 2020;8:272
Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip.
Jalili-Firoozinezhad S, Prantil-Baun R, Jiang A, Potla R, Mammoto T, Weaver JC, Ferrante TC, Kim HJ, Cabral JMS, Levy O, Ingber DE
Cell death & disease 2018 Feb 14;9(2):223
Cell death & disease 2018 Feb 14;9(2):223
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Villin in methanol-fixed Hep3B cells using a Villin polyclonal antibody (Product # PA5-29078) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Villin (Product # PA5-29078) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 Evaluation of intestinal epithelial glycocalyx and microvilli. (A) Confocal immunefluorescence views of the sugar chain portion (FITC-WGA, green) of the glycocalyx layer secreted by the Caco2 cells cultured in the static Transwell system, or in the peristaltic microfluidic chip in the absence (Peris) or presence (Peris + EC) of endothelial cell for 5 days (bar, 50 mum). Computerized quantification of the coverage (B) and height (C) of the sugar chain portion marked by FITC-labeled WGA measured under the conditions described in (A) ( n = 3; * P < 0.05, ** p < 0.01, *** P < 0.001, **** P < 0.0001). (D) Confocal immunofluorescence views of the villin (red) of the microvilli secreted by the Caco2 cells cultured in the static Transwell system, or in the peristaltic microfluidic chip in the absence (Peris) or presence (Peris + EC) of endothelial cell for 5 days (bar, 50 mum). Quantification of the coverage (E) and height (F) of villin done under the conditions as described in (D) ( n = 3; ** p < 0.01, **** P < 0.0001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 7 Intestinal damage and inflammatory responses caused by E. coli on the chip. (A) Conceptual diagram of the microsystem of human inflammatory bowel disease representing co-culture of vascular endothelial cells, enterocyte, macrophages with enterobacteria. (B) The confocal fluorescence views showing the distribution of the tight junction protein - occludin (green) in the epithelial monolayers cultured in the peristaltic human gut-vessel microsystem under control conditions (EC) versus presence of E. coli with (EC + E. coli ) or without ( E. coli ) endothelial cells for 24 h (bar, 50 mum). (C) Quantification of Papp measured by quantitating fluorescent dextran transport across intestinal epithelial layer under the conditions as described in (B) ( n = 3; * P < 0.05, ** p < 0.01, *** P < 0.001). (D) Confocal immunofluorescence views of the sugar chain portion (FITC-WGA, green) of the glycocalyx layer secreted by the Caco2 cells cultured under the conditions described in (B) (nuclei in blue, E. coli in red) (bar, 50 mum). (E) Confocal immunofluorescence views of the villin of the microvilli secreted by the Caco2 cells cultured under the conditions described in (B) (nuclei in blue, villin in red) (bar, 50 mum). Computerized quantification of the coverage (F) and height (G) of the sugar chain portion and villin measured under the conditions described in (B) ( n = 3; ** p < 0.01, *** P < 0.001, **** P < 0.0001). Polarized secretion of proinflammatory cytokines (IL-8, IL-6, TNF
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 8 Anti-inflammatory evaluation of L. casei and antibiotics on the chip. (A) The confocal fluorescence views showing the distribution of the tight junction protein - occludin (green) in the epithelial monolayers cultured in the peristaltic human gut-vessel microsystem with E. coli under control conditions (EC + E. coli ) versus presence of L. casei (EC + E. coli + L. casei ) or P/S (EC + E. coli + P/S) for 24 h (bar, 50 mum). (B) Quantification of Papp measured by quantitating fluorescent dextran transport across intestinal epithelial layer under the conditions described in (A) ( n = 3; * P < 0.05, ** p < 0.01). (C) Confocal immunofluorescence views of the sugar chain portion (FITC-WGA, green) of the glycocalyx layer secreted by the Caco2 cells cultured under the conditions described in (A) (nuclei in blue, E. coli in red) (bar, 50 mum). (D) Confocal immunofluorescence views of the villin of the microvilli layer secreted by the Caco2 cells cultured under the conditions described in (A) (nuclei in blue, villin in red) (bar, 50 mum). Computerized quantification of the coverage (E) and height (F) of the sugar chain portion and villin measured under the conditions described in (A) ( n = 3; ** p < 0.01, *** P < 0.001). Polarized secretion of proinflammatory cytokines (IL-8, IL-6, TNF-alpha, and IL-1beta) in the enteric cavity (H) and vessel lumen (G) under the conditions described in (A) ( n = 3; * P < 0.05, ** p < 0.01).