- PA5-29078 - Invitrogen Antibodies VIL1 antibody

Submitted references Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.

Jing B, Wang ZA, Zhang C, Deng Q, Wei J, Luo Y, Zhang X, Li J, Du Y
Frontiers in bioengineering and biotechnology 2020;8:272

Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip.

Jalili-Firoozinezhad S, Prantil-Baun R, Jiang A, Potla R, Mammoto T, Weaver JC, Ferrante TC, Kim HJ, Cabral JMS, Levy O, Ingber DE
Cell death & disease 2018 Feb 14;9(2):223

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of Villin was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Villin Polyclonal Antibody (Product # PA5-29078) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Villin Polyclonal Antibody (Product # PA5-29078). Sample (30 µg of whole cell lysate). Lane A: H1299 . 7.5% SDS PAGE. Villin Polyclonal Antibody (Product # PA5-29078) diluted at 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Villin Polyclonal Antibody (Product # PA5-29078) and a 92kDa band corresponding to Villin was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), COLO 205 (Lane 2), HT-29 (Lane 3), Jurkat (Lane 4) and PANC-1 (Lane 5) as seen in Fig (a).Tissue extracts of Mouse Liver (Lane 1), Mouse Colon (Lane 2) and Mouse Jejunum (Lane 3) as seen in Fig (b) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Villin in methanol-fixed Hep3B cells using a Villin polyclonal antibody (Product # PA5-29078) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Villin (Product # PA5-29078) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 Evaluation of intestinal epithelial glycocalyx and microvilli. (A) Confocal immunefluorescence views of the sugar chain portion (FITC-WGA, green) of the glycocalyx layer secreted by the Caco2 cells cultured in the static Transwell system, or in the peristaltic microfluidic chip in the absence (Peris) or presence (Peris + EC) of endothelial cell for 5 days (bar, 50 mum). Computerized quantification of the coverage (B) and height (C) of the sugar chain portion marked by FITC-labeled WGA measured under the conditions described in (A) ( n = 3; * P < 0.05, ** p < 0.01, *** P < 0.001, **** P < 0.0001). (D) Confocal immunofluorescence views of the villin (red) of the microvilli secreted by the Caco2 cells cultured in the static Transwell system, or in the peristaltic microfluidic chip in the absence (Peris) or presence (Peris + EC) of endothelial cell for 5 days (bar, 50 mum). Quantification of the coverage (E) and height (F) of villin done under the conditions as described in (D) ( n = 3; ** p < 0.01, **** P < 0.0001).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 7 Intestinal damage and inflammatory responses caused by E. coli on the chip. (A) Conceptual diagram of the microsystem of human inflammatory bowel disease representing co-culture of vascular endothelial cells, enterocyte, macrophages with enterobacteria. (B) The confocal fluorescence views showing the distribution of the tight junction protein - occludin (green) in the epithelial monolayers cultured in the peristaltic human gut-vessel microsystem under control conditions (EC) versus presence of E. coli with (EC + E. coli ) or without ( E. coli ) endothelial cells for 24 h (bar, 50 mum). (C) Quantification of Papp measured by quantitating fluorescent dextran transport across intestinal epithelial layer under the conditions as described in (B) ( n = 3; * P < 0.05, ** p < 0.01, *** P < 0.001). (D) Confocal immunofluorescence views of the sugar chain portion (FITC-WGA, green) of the glycocalyx layer secreted by the Caco2 cells cultured under the conditions described in (B) (nuclei in blue, E. coli in red) (bar, 50 mum). (E) Confocal immunofluorescence views of the villin of the microvilli secreted by the Caco2 cells cultured under the conditions described in (B) (nuclei in blue, villin in red) (bar, 50 mum). Computerized quantification of the coverage (F) and height (G) of the sugar chain portion and villin measured under the conditions described in (B) ( n = 3; ** p < 0.01, *** P < 0.001, **** P < 0.0001). Polarized secretion of proinflammatory cytokines (IL-8, IL-6, TNF

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 8 Anti-inflammatory evaluation of L. casei and antibiotics on the chip. (A) The confocal fluorescence views showing the distribution of the tight junction protein - occludin (green) in the epithelial monolayers cultured in the peristaltic human gut-vessel microsystem with E. coli under control conditions (EC + E. coli ) versus presence of L. casei (EC + E. coli + L. casei ) or P/S (EC + E. coli + P/S) for 24 h (bar, 50 mum). (B) Quantification of Papp measured by quantitating fluorescent dextran transport across intestinal epithelial layer under the conditions described in (A) ( n = 3; * P < 0.05, ** p < 0.01). (C) Confocal immunofluorescence views of the sugar chain portion (FITC-WGA, green) of the glycocalyx layer secreted by the Caco2 cells cultured under the conditions described in (A) (nuclei in blue, E. coli in red) (bar, 50 mum). (D) Confocal immunofluorescence views of the villin of the microvilli layer secreted by the Caco2 cells cultured under the conditions described in (A) (nuclei in blue, villin in red) (bar, 50 mum). Computerized quantification of the coverage (E) and height (F) of the sugar chain portion and villin measured under the conditions described in (A) ( n = 3; ** p < 0.01, *** P < 0.001). Polarized secretion of proinflammatory cytokines (IL-8, IL-6, TNF-alpha, and IL-1beta) in the enteric cavity (H) and vessel lumen (G) under the conditions described in (A) ( n = 3; * P < 0.05, ** p < 0.01).

PA5-29078

Antibody data