Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AP32450PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti IRS4
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide corresponding to amino acids 1240-1257 of Human IRS-4. The immunizing sequence does not share any significant homology with IRS-1, IRS-2 or IRS-3.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Immunoblot Analysis: HEK cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-rabbit IRS-4 (1μg/ml). Proteins were visualized using a goat anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Lane 1 shows preincubation of the IRS-4 antibody with 10μM of the immunizing peptide. Lane 2: shows the immunoreactivity in the absence of the immunizing peptide.