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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-117329 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRS4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Centrifuge briefly prior to opening.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRS4 in Various whole cell extracts (30 µg). Samples were separated with 5% SDS-PAGE and incubated in IRS4 polyclonal antibody (Product # PA5-117329) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IRS4 in Various whole cell extracts (30 µg). Samples were separated with 5% SDS-PAGE and incubated in IRS4 polyclonal antibody (Product # PA5-117329) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IRS4 in 4% paraformaldehyde (RT, 15 min) fixed 293T cells (Green: IRS4). Samples was incubated in IRS4 polyclonal antibody (Product # PA5-117329) using a dilution of 1:500, followed by Hoechst.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IRS4 in paraffin-embedded human hepatocellular carcinoma. Samples was treated with Citrate buffer (pH 6, 15 min) for antigen retrieval and incubated in IRS4 polyclonal antibody (Product # PA5-117329) using a dilution of 1:500.
