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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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- Product number
- PA5-31078 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOXC10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, mouse muscle. Predicted reactivity: Mouse (97%), Rat (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references HAND1 and BARX1 Act as Transcriptional and Anatomic Determinants of Malignancy in Gastrointestinal Stromal Tumor.
Hemming ML, Coy S, Lin JR, Andersen JL, Przybyl J, Mazzola E, Abdelhamid Ahmed AH, van de Rijn M, Sorger PK, Armstrong SA, Demetri GD, Santagata S
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Mar 15;27(6):1706-1719
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Mar 15;27(6):1706-1719
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Homeobox C10 using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Homeobox C10 polyclonal antibody (Product # PA5-31078) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HOXC10 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a HOXC10 Polyclonal Antibody (Product # PA5-31078) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HOXC10 Polyclonal Antibody (Product # PA5-31078). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HOXC10 Polyclonal Antibody (Product # PA5-31078) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HOXC10 Polyclonal Antibody detects Homeobox C10 protein by western blot analysis. A.50 µg mouse muscle lysate/extract.10% SDS-PAGE. HOXC10 Polyclonal Antibody (Product # PA5-31078) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Homeobox C10 in paraformaldehyde-fixed HeLa cells using a Homeobox C10 polyclonal antibody (Product # PA5-31078) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HOXC10 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HOXC10 Polyclonal Antibody (Product # PA5-31078) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
