Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-3179-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD317 (BST2, PDCA-1) Monoclonal Antibody (26F8), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 26F8 monoclonal antibody reacts with human CD317 (also known as BST2 and tetherin). CD317 is a 30-36-kDa type II transmembrane protein expressed on B cells and bone marrow stromal cells. Although reports have indicated that CD317 mRNA is detectable in activated T cells, protein expression in freshly isolated primary T cells and macrophages is undetectable. Moreover, certain T cell lines, such as Jurkat, do not express detectable levels of CD317 protein. However, CD317 expression can be induced in many cell types (e.g., T and B cells, 293T, and HeLa) by IFNalpha treatment. CD317 has been associated with pre-B cell growth and the terminal differentiation of plasma B cells. More recently, this molecule has been reported to prevent HIV-1 virion release from the surface of infected cells, leading to reuptake and degradation of the virus. This activity is inhibited by the HIV-1 accessory protein Vpu. CD317 has been identified as the ligand for the ILT7 receptor. Applications Reported: This 26F8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 26F8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 26F8
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references CD317 is over-expressed in B-cell chronic lymphocytic leukemia, but not B-cell acute lymphoblastic leukemia.
Nef proteins of epidemic HIV-1 group O strains antagonize human tetherin.
Ig-like transcript 7, but not bone marrow stromal cell antigen 2 (also known as HM1.24, tetherin, or CD317), modulates plasmacytoid dendritic cell function in primary human blood leukocytes.
Vpu enhances HIV-1 virus release in the absence of Bst-2 cell surface down-modulation and intracellular depletion.
Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu.
The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein.
Molecular cloning and chromosomal mapping of a bone marrow stromal cell surface gene, BST2, that may be involved in pre-B-cell growth.
Gong S, Osei ES, Kaplan D, Chen YH, Meyerson H
International journal of clinical and experimental pathology 2015;8(2):1613-21
International journal of clinical and experimental pathology 2015;8(2):1613-21
Nef proteins of epidemic HIV-1 group O strains antagonize human tetherin.
Kluge SF, Mack K, Iyer SS, Pujol FM, Heigele A, Learn GH, Usmani SM, Sauter D, Joas S, Hotter D, Bibollet-Ruche F, Plenderleith LJ, Peeters M, Geyer M, Sharp PM, Fackler OT, Hahn BH, Kirchhoff F
Cell host & microbe 2014 Nov 12;16(5):639-50
Cell host & microbe 2014 Nov 12;16(5):639-50
Ig-like transcript 7, but not bone marrow stromal cell antigen 2 (also known as HM1.24, tetherin, or CD317), modulates plasmacytoid dendritic cell function in primary human blood leukocytes.
Tavano B, Galao RP, Graham DR, Neil SJ, Aquino VN, Fuchs D, Boasso A
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2622-30
Journal of immunology (Baltimore, Md. : 1950) 2013 Mar 15;190(6):2622-30
Vpu enhances HIV-1 virus release in the absence of Bst-2 cell surface down-modulation and intracellular depletion.
Miyagi E, Andrew AJ, Kao S, Strebel K
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2868-73
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 24;106(8):2868-73
Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu.
Neil SJ, Zang T, Bieniasz PD
Nature 2008 Jan 24;451(7177):425-30
Nature 2008 Jan 24;451(7177):425-30
The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein.
Van Damme N, Goff D, Katsura C, Jorgenson RL, Mitchell R, Johnson MC, Stephens EB, Guatelli J
Cell host & microbe 2008 Apr 17;3(4):245-52
Cell host & microbe 2008 Apr 17;3(4):245-52
Molecular cloning and chromosomal mapping of a bone marrow stromal cell surface gene, BST2, that may be involved in pre-B-cell growth.
Ishikawa J, Kaisho T, Tomizawa H, Lee BO, Kobune Y, Inazawa J, Oritani K, Itoh M, Ochi T, Ishihara K
Genomics 1995 Apr 10;26(3):527-34
Genomics 1995 Apr 10;26(3):527-34
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were treated with human IFN alpha 2 and then stained with Anti-Human CD19 (HIB19) APC (Product # 17-0199-42) and Mouse IgG1, K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD317 (BST2, PDCA-1) PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye