Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000684-M15 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000684-M15, RRID:AB_1502753
- Product name
- Mouse Monoclonal BST2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. BST2 - bone marrow stromal cell antigen 2 (2E6)
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway.
Epitope tags beside the N-terminal cytoplasmic tail of human BST-2 alter its intracellular trafficking and HIV-1 restriction.
Purification of eukaryotic tetherin/Vpu proteins and detection of their interaction by ELISA.
Polarity changes in the transmembrane domain core of HIV-1 Vpu inhibits its anti-tetherin activity.
Liu Y, Li M, Zhang D, Zhang M, Hu Q
Virology journal 2016 Sep 15;13(1):154
Virology journal 2016 Sep 15;13(1):154
Epitope tags beside the N-terminal cytoplasmic tail of human BST-2 alter its intracellular trafficking and HIV-1 restriction.
Lv M, Wang J, Zhang J, Zhang B, Wang X, Zhu Y, Zuo T, Liu D, Li X, Wu J, Zhang H, Yu B, Wu H, Zhao X, Kong W, Yu X
PloS one 2014;9(10):e111422
PloS one 2014;9(10):e111422
Purification of eukaryotic tetherin/Vpu proteins and detection of their interaction by ELISA.
Lv M, Zhu Y, Wang J, Zhang H, Wang X, Zuo T, Liu D, Zhang J, Wu J, Kong W, Yu X
Protein expression and purification 2013 Oct;91(2):112-8
Protein expression and purification 2013 Oct;91(2):112-8
Polarity changes in the transmembrane domain core of HIV-1 Vpu inhibits its anti-tetherin activity.
Lv M, Wang J, Wang X, Zuo T, Zhu Y, Kong W, Yu X
PloS one 2011;6(6):e20890
PloS one 2011;6(6):e20890
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BST2 Antibody (2E6) [H00000684-M15] - Analysis of BST2 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: BST2 Antibody (2E6) [H00000684-M15] - Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: BST2 Antibody (2E6) [H00000684-M15] - Analysis of monoclonal antibody to BST2 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BST2 Antibody (2E6) [H00000684-M15] - Analysis of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BST2 rabbit polyclonal antibody.
