Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-80385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BST-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of BST-2 in Lane A: Hela Whole Cell Lysate (30 µg), Lane B: Jurkat Whole Cell Lysate (30 µg), Lane C: 293 Whole Cell Lysate (30 µg). Samples were probed using a BST-2 Polyclonal Antibody (Product # PA5-80385) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:20 kDa. Observed band size:28 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BST-2 Polyclonal Antibody (Product # PA5-80385) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: 293 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 28 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-BST-2 Polyclonal Antibody (Product # PA5-80385) and a ~30 kDa and ~55 kDa band corresponding to Bone marrow stromal antigen 2 was observed in HeLa. Whole cell extracts (30 µg lysate) of HeLa (Lane 1) and IFNa treated HeLa (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). https://doi.org/10.1038/cddis.2017.68.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Bone marrow stromal antigen 2 was performed using 70% confluent log phase HeLa cells treated with 1 ng/mL IFNa for 24 Hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BST-2 Polyclonal Antibody (Product # PA5-80385) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nucleus and cytoplasm localization. Panel e represents untreated cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human BST-2 in human kidney with BST-2 Polyclonal Antibody (Product # PA5-80385, 1:5,000 dilution, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human BST-2 in human liver with BST-2 Polyclonal Antibody (Product # PA5-80385, 1:5,000 dilution, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- BST-2 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate 1 µL with BST-2 Polyclonal Antibody (Product # PA5-80385) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: BST-2 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 28 kDa.