PA5-92999

antibody from Invitrogen Antibodies

Targeting: POLE3 CHARAC17, CHRAC17, CHRAC2, p17, Ybl1

Antibody data

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Validation data

Product number: PA5-92999 - Provider product page
Provider: Invitrogen Antibodies
Product name: POLE3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS CANNFAMKGK RKTLNASDVL SAMEEMEFQR FVTPLKEALE AYRREQKGKK EASEQKKKDK DKKTDSEEQD KSRDEDNDED EERLEEEEQN EEEEVDN; Positive Samples: HepG2, Mouse testis, Mouse bone marrow, Mouse spleen; Cellular Location: Nucleus
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.44 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

POLE3 protein structure - PA5-92999 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts of various cell lines, using POLE3 Polyclonal antibody (Product # PA5-92999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts from normal (control) and POLE3 knockout (KO) 293T cells, using POLE3 Polyclonal antibody (Product # PA5-92999) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of POLE3 in extracts from normal (control) and POLE3 knockout (KO) 293T cells using POLE3 Polyclonal Antibody (Product # PA5-92999) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of POLE3 in extracts of various cell lines using POLE3 Polyclonal Antibody (Product # PA5-92999) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.