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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA1-16509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLE3 Monoclonal Antibody (3C5.1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody does not detect drosophilia or the baculovirus host cell pol epsilon, and weakly, if at all, on s.cerivisae pol epsilon.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3C5.1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLE3 in HeLa DNA. Samples were incubated in POLE3 monoclonal antibody (Product # MA1-16509). The Mono S fraction (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of POLE3 in formalin-fixed paraffin-embedded tissue section of human kidney. Samples were incubated in POLE3 monoclonal antibody (Product # MA1-16509) using a dilution of 1:200 followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin.
