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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-30743 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLE3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, POLE3 shRNA-transfected 293T. Predicted reactivity: Mouse (95%), Rat (95%), Zebrafish (81%), Xenopus laevis (90%), Chicken (90%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.01 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DNA Polymerase epsilon in paraformaldehyde-fixed HeLa cells using a DNA Polymerase epsilon polyclonal antibody (Product # PA5-30743) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNA polymerase epsilon (Product # PA5-30743) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNA polymerase epsilon antibody [N1C3] detects DNA Polymerase epsilon protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. DNA Polymerase epsilon antibody [N1C3] (Product # PA5-30743) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
