Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- AF3154 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Sortilin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Sortilin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references proBDNF is modified by advanced glycation end products in Alzheimer's disease and causes neuronal apoptosis by inducing p75 neurotrophin receptor processing.
Transcriptional control of intestinal cholesterol absorption, adipose energy expenditure and lipid handling by Sortilin.
Lack of human-like extracellular sortilin neuropathology in transgenic Alzheimer's disease model mice and macaques.
Role of endogenous brain-derived neurotrophic factor and sortilin in B cell survival.
Role of endogenous brain-derived neurotrophic factor and sortilin in B cell survival.
Fleitas C, Piñol-Ripoll G, Marfull P, Rocandio D, Ferrer I, Rampon C, Egea J, Espinet C
Molecular brain 2018 Nov 14;11(1):68
Molecular brain 2018 Nov 14;11(1):68
Transcriptional control of intestinal cholesterol absorption, adipose energy expenditure and lipid handling by Sortilin.
Hagita S, Rogers MA, Pham T, Wen JR, Mlynarchik AK, Aikawa M, Aikawa E
Scientific reports 2018 Jun 13;8(1):9006
Scientific reports 2018 Jun 13;8(1):9006
Lack of human-like extracellular sortilin neuropathology in transgenic Alzheimer's disease model mice and macaques.
Zhou FQ, Jiang J, Griffith CM, Patrylo PR, Cai H, Chu Y, Yan XX
Alzheimer's research & therapy 2018 Apr 24;10(1):40
Alzheimer's research & therapy 2018 Apr 24;10(1):40
Role of endogenous brain-derived neurotrophic factor and sortilin in B cell survival.
Fauchais AL, Lalloué F, Lise MC, Boumediene A, Preud'homme JL, Vidal E, Jauberteau MO
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 1;181(5):3027-38
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 1;181(5):3027-38
Role of endogenous brain-derived neurotrophic factor and sortilin in B cell survival.
Fauchais AL, Lalloué F, Lise MC, Boumediene A, Preud'homme JL, Vidal E, Jauberteau MO
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 1;181(5):3027-38
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 1;181(5):3027-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Sortilin by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 82 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Sortilin by Western Blot. Western blot shows lysates of human motor cortex. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sortilin in Human Brain. Sortilin was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Sortilin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Sortilin in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human Sortilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3154, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.