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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-47462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Sortilin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human motor cortex. PVDF membrane was probed with 2 µg/mL of Goat Anti-human Sortilin Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47462) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human motor cortex. PVDF membrane was probed with 2 µg/mL of Goat Anti-human Sortilin Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47462) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Sortilin in human motor cortex. Samples were incubated in Sortilin polyclonal antibody (Product # PA5-47462) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Sortilin in 0.2 mg/mL lysates of human brain (cerebellum) tissue. Samples were incubated in Sortilin polyclonal antibody (Product # PA5-47462) at a dilution of 10 µg/mL. A specific band was detected for Sortilin at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Sortilin Polyclonal Antibody (Product # PA5-47462) and a 110kDa band corresponding to SORT1 was observed across all cell lines and tissues tested except in Mouse and Rat liver. Whole cell extracts (30 µg lysate) of SK-BR-3 (Lane 1), MDA-MB-231 (Lane 2), BeWo (Lane 3), HeLa (Lane 4), Mouse brain (Lane 5), Rat brain (Lane 6), Mouse liver (Lane 7), Rat liver (Lane 8) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Sortilin in immersion fixed paraffin-embedded sections of human brain. Samples were incubated in Sortilin polyclonal antibody (Product # PA5-47462) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Sortilin in K562 human chronic myelogenous leukemia cell line. Samples were incubated in Sortilin polyclonal antibody (Product # PA5-47462) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Sortilin in Human peripheral blood monocytes. Samples were incubated in Sortilin polyclonal antibody (Product # PA5-47462) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
