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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANT-009-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Sortilin (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: - 1,2. Anti-Sortilin (extracellular) Antibody (#ANT-009), (1:400).3,4. Anti-Sortilin (extracellular) Antibody , preincubated with Sortilin (extracellular) Blocking Peptide (#BLP-NT009).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: - 1,2. Anti-Sortilin (extracellular) Antibody (#ANT-009), (1:400).3,4. Anti-Sortilin (extracellular) Antibody , preincubated with Sortilin (extracellular) Blocking Peptide (#BLP-NT009).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Sortilin in rat neocortex - Immunohistochemical staining of rat neocortex with Anti-Sortilin (extracellular) Antibody (#ANT-009). A. Sortilin appears in cortical neurons (red). B. Staining of interneurons with mouse Anti-parvalbumin (PV, green). C. Confocal merge of Sortilin and PV demonstrates separate localization in neocortex.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Indirect flow cytometry of live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Sortilin (extracellular) Antibody (#ANT-009), (2.5 µg) + goat- Anti-rabbit-FITC.
