Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29195 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Sortilin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Mouse brain. Predicted reactivity: Mouse (89%), Rat (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Sortilin 1 using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Sortilin 1 polyclonal antibody (Product # PA5-29195) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Sortilin Polyclonal Antibody (Product # PA5-29195). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. Sortilin Polyclonal Antibody (Product # PA5-29195) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Sortilin was performed in DIV10 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: Sortilin Polyclonal Antibody (Product # PA5-29195) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Sortilin was performed in paraffin-embedded human breast carcinoma tissue using Sortilin Polyclonal Antibody (Product # PA5-29195) at a dilution of 1:500.