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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005998-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005998-M01, RRID:AB_425656
- Product name
- RGS3 monoclonal antibody (M01), clone 1E8-C7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant RGS3.
- Antigen sequence
MLRGMYLTRNGNLQRRHTMKEAKDMKNKLGIFRRR
SESPGAPPAGKADKMMKSFKPTSEEALKWGESLEK
LLVHKYGLAVFQAFLRTEFSEENLEFWLACEDFKK
VKSQSKMASKAKKIFAEYIAIQACKEVNLDSYTRE
HTKDNLQSVTRGCFDLAQKRIFGLMEKDSYPRFLR
SDLYLDLINQKKMSPPL- Isotype
- IgG
- Antibody clone number
- 1E8-C7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RGS3 expression in transfected 293T cell line by RGS3 monoclonal antibody (M01), clone 1E8-C7.Lane 1: RGS3 transfected lysate(19.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RGS3 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to RGS3 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to RGS3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
