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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- BS-15353R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPCR EX33 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse Spinal Cord lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated (bs-15353R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded human glioma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded human colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (bs-15353R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
