Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- NB500-352PE - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Cytokeratin 7/17 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [PE] [NB500-352PE] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 (C-46) antibody NB500-352PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [PE] [NB500-352PE] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 antibody (C-46) NB500-352PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.