Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX109723 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109723, RRID:AB_1950654
- Product name
- Cytokeratin 7 antibody [N1C2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.
TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.
Endoglin Is Essential for the Maintenance of Self-Renewal and Chemoresistance in Renal Cancer Stem Cells.
DaSilva-Arnold SC, Kuo CY, Davra V, Remache Y, Kim PCW, Fisher JP, Zamudio S, Al-Khan A, Birge RB, Illsley NP
Molecular human reproduction 2019 Feb 1;25(2):61-75
Molecular human reproduction 2019 Feb 1;25(2):61-75
TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
Pei YF, Yin XM, Liu XQ
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207
Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.
DaSilva-Arnold SC, Zamudio S, Al-Khan A, Alvarez-Perez J, Mannion C, Koenig C, Luke D, Perez AM, Petroff M, Alvarez M, Illsley NP
Biology of reproduction 2018 Aug 1;99(2):409-421
Biology of reproduction 2018 Aug 1;99(2):409-421
Endoglin Is Essential for the Maintenance of Self-Renewal and Chemoresistance in Renal Cancer Stem Cells.
Hu J, Guan W, Liu P, Dai J, Tang K, Xiao H, Qian Y, Sharrow AC, Ye Z, Wu L, Xu H
Stem cell reports 2017 Aug 8;9(2):464-477
Stem cell reports 2017 Aug 8;9(2):464-477
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: A431 (GTX27909)10% SDS PAGEGTX109723 diluted at 1:10000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2] (GTX109723) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2] (GTX109723) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of human placenta (paraformaldehyde-fixed frozen sections), using Cytokeratin 7(GTX109723) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 7(GTX109723) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4¢J and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.