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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX110414 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110414, RRID:AB_11164456
- Product name
- Cytokeratin 7 antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references KRas4B-PDE6δ complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer.
Casique-Aguirre D, Briseño-Díaz P, García-Gutiérrez P, la Rosa CHG, Quintero-Barceinas RS, Rojo-Domínguez A, Vergara I, Medina LA, Correa-Basurto J, Bello M, Hernández-Rivas R, Del RocioThompson-Bonilla M, Vargas M
BMC cancer 2018 Dec 29;18(1):1299
BMC cancer 2018 Dec 29;18(1):1299
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX110414 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 7(GTX110414) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Cytokeratin 7(GTX110414) antibody at 1:500 dilution.
