PA5-34764

antibody from Invitrogen Antibodies

Targeting: KRT7 CK7, K2C7, K7, SCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34764

Provider

Invitrogen Antibodies

Product name

Cytokeratin 7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa.

Concentration

0.78 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cytokeratin 7 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Cytokeratin 7 polyclonal antibody (Product # PA5-34764) at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Cytokeratin 7 Polyclonal Antibody (Product # PA5-34764). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 Polyclonal Antibody (Product # PA5-34764) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Cytokeratin 7 Polyclonal Antibody (Product # PA5-34764) and a 51 kDa band corresponding to KRT-7 was observed in positive cell lines HeLa, A549, OVCAR-3, PC-3 and not in negative cell lines SK-OV-3 as mentioned in literature. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), OVCAR-3 (Lane 3), SK-OV-3 (Lane 4) and PC-3 (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cytokeratin 7 in methanol-fixed HeLa cells using a Cytokeratin 7 polyclonal antibody (Product # PA5-34764) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Cytokeratin 7 (Product # PA5-34764) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.