NB500-352

antibody from Novus Biologicals

Targeting: KRT7 CK7, K2C7, K7, SCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB500-352

Provider

Novus Biologicals

Proper citation

Novus Cat#NB500-352, RRID:AB_10001529

Product name

Mouse Monoclonal Cytokeratin 7/17 Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.

Reactivity

Human, Mouse, Rat, Bovine, Porcine, Rabbit

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Detection of Cytokeratin 7+17 in HeLa cell lysate with monoclonal antibody C-46.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Fig. 1. Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7/17 Antibody (C-46).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46).

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 antibody (C-46) NB500-352PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Cytokeratin 7/17 Antibody (C-46) [NB500-352] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on A549 cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): Cytokeratin 7/17 Antibody (C-46) [NB500-352] - An intracellular stain was performed on HeLa cells with Cytokeratin 7/17 (C-46) antibody NB500-352AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.