601601

antibody from BioLegend

Targeting: KRT7 CK7, K2C7, K7, SCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

601601

Provider

BioLegend

Proper citation

BioLegend Cat#601601, RRID:AB_2715904

Product name

Purified anti-human Cytokeratin 7

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16155A

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate (15ug protein) from HaCaT, A549, MCF-7 (negative control) and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-KRT7 antibody (clone W16155A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (clone Poly4054) for clone W16155A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP (clone Poly4064) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder, M* indicates longer exposure.

Conjugate

Unconjugated