Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 601602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#601602, RRID:AB_2715905
- Product name
- Purified anti-human Cytokeratin 7
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W16155A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Total cell lysate (15ug protein) from HaCaT, A549, MCF-7 (negative control) and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-KRT7 antibody (clone W16155A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (clone Poly4054) for clone W16155A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP (clone Poly4064) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder, M* indicates longer exposure.
- Conjugate
- Unconjugated